This article presents the Night of the Churches as one of the possible and effective responses to the challenges discussed in Pope John Paul II’s 2003 post-synodal apostolic exhortation Ecclesia in Europa, and introduces the history, spread and purpose of this activity. It focuses in detail on its implementation in the Czech Republic since 2009, with special emphasis on the years 2000—2002, marked by the pandemic of COVID-19. It also tries to show its positives (chances) and negatives (pitfalls). In this way, it can serve not only as a recapitulation of past actions, but also as an inspiration, especially for countries where this activity is not widespread and often not even known.