In the academic year 2023/2024, Polish studies in Czech universities celebrated its 100th anniversary. In view of this important anniversary, it is worth outlining the history of the field, as well as its transformation and current status. The roots of Polish Studies interest in the Czech Republic go back to the late Enlightenment, i.e. the beginning of the Czech national revival. On university grounds, i.e. at Prague University, Polish studies emerged in the mid-19th century. The breakthrough moment, however, was the establishment of an independent Department of Polish Language and Literature in the academic year 1923/1924 at the Faculty of Philosophy of Charles University and Polish language lectureships at the universities in Brno and Bratislava. The direction, which was largely part of the unified Slavonic studies, was represented here for many years in the form of a foreign language course, to which classes in grammar, literary history, Slavic comparative studies and history were linked. However, the disintegration of the former Slavonic studies in the 1950s had negative consequences for the development of individual Slavonic philologies including Polish studies. After the political changes in Czechoslovakia, a long-term process of modernisation of the field of study took place from the 1990s onwards. It concerned the structure of studies, didactic methods and the enrichment of the offer of subjects in the fields of translation studies, business Polish, Central European issues, as well as law, economics and tourism. After 30 years of implementing the above-mentioned changes, Czech Polish studies has taken on the character of a broad-based Polish studies programme. A graduate of Polish studies is now not only an expert in Polish language and literature but also a specialist in the knowledge of Poland, its culture, economic and tourism potential and Czech-Polish relations.